Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEHYFYVLFCK--DQTFYGGYTTDLSRRLEEHNNGT--GAKYTRLQK--RRPVKMIHA-EVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI 4XM5 Chain:A ((8-117)) -PDFYGCYLLQSISKRQSFYIGSTPNPVRRLRQHNGSLSR--------RDGTRPWEMVAIVYGFPSRIAALQFQHAWQHGYQTR-----------------

General information: TITO was launched using: RESULT: Template: 4XM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 250 -5431 -21.72 -79.86 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -21.72 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.640

(partial model without unconserved sides chains): PDB file : Tito_4XM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XM5-query.scw PDB file : Tito_Scwrl_4XM5.pdb: