Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEAVEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDELPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAYRLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEAISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQENGVTKLVKKSN 4EGZ Chain:B ((52-82)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELMQQFGVSRHTIRKAIGDLVSQGLLYSVQG---------

General information: TITO was launched using: RESULT: Template: 4EGZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 43 -8742 -203.29 -281.98 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -203.29 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.800

(partial model without unconserved sides chains): PDB file : Tito_4EGZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGZ-query.scw PDB file : Tito_Scwrl_4EGZ.pdb: