Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTAGAKSATYFFHK---TTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG 5SVA Chain:T ((37-127)) ---LSKVQLYEDLCRYEDTLSKL---VESVDRFKPNLDIAKDLIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNECHDELKA

General information: TITO was launched using: RESULT: Template: 5SVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 103 -2092 -20.31 -23.77 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain T : 0.57 3D Compatibility (PKB) : -20.31 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_5SVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5SVA-query.scw PDB file : Tito_Scwrl_5SVA.pdb: