Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQ-----PKQYEYSRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHA-VFSLFQAGDHILLGDDVYGGTFRLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLAHQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQIGF--YQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPGLPDHELHGVAKQQMSGFSGMISFTLKNE-ESAIPFVESLQLFTLAESLGGVESLVEIPSVMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN 5K30 Chain:A ((11-395)) ----TQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARF-------RLGNPTTDALEKKLAVLERGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYG-THAFLSHSMPKFGINVSFVDAAKPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDNTFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGK-QEFIDQARFVGLKDITGG-MSPFNAWLTLRGVKTLGIRMERHCENALKIARFLEGHPSITRVYYPGLSSHPQYELGQRQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEERLKAGITDGLIRLSVGLEDPEDIINDLEHAIRK---

General information: TITO was launched using: RESULT: Template: 5K30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2102 50020 23.80 137.42 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 23.80 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_5K30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K30-query.scw PDB file : Tito_Scwrl_5K30.pdb: