Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMDVKAYILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGTSAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRPTVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKNK 5GAD Chain:C ((103-271)) -------YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRN-FG-KHPVTPWGVQTKGKKTRSNK-RTDKFIVRRR---

General information: TITO was launched using: RESULT: Template: 5GAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 616 -40297 -65.42 -238.44 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.85 3D Compatibility (PKB) : -65.42 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_5GAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAD-query.scw PDB file : Tito_Scwrl_5GAD.pdb: