Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVSAFSSAQKMVGSINSFSLI-AIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTNVLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGM---VIPPSTAFI---MYSLVA-------GGASISALFMGGYLVGALWALGIMLVAFIVAKKN-KYP-VVEK-G---QMAHAGKIIKEAIPSLLLIVIIIGGIL-TGI-----------FTAVEASAIAVIYSLLIAMFY--YK----TV-KLKDIPNMLFQSVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGV-T---DNKFIILLLVNLVLLLVGMFM-DVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVG-SITPPVGTGLYVGASVGG-----VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS 4R0C Chain:C ((70-480)) ------------------------------------------------FTTLVGNFTGFAPLGVVLVAMLGLGVAEQSGLLSVSLASLVRRSSGGAL-V--FTVAFAGVLSSL-TV-DAGYVVLIPLAGLVFQLAGRPPIAGIATAFAAVSGGFSANLLVGPVDATLAGLSTEAAHIIDPDRT-VAATGNYWFIIASTFLVTGLVT-LITRTLTEPRLAHANTVADASVDAPQIHSRAMKWTGLTLAILLAGLALLVLPNDAPLRHPDTGSVLGSPFIHGLVVIVALIAGICGAVYGRVSGQFRNSGAVITAMEVTMASMAGYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLMLLGISPEASQAAYRVGDSSTNIITPLMP-YFVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWSVLL--------------

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1723 -264942 -153.77 -733.91 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -153.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: