Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTHNDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQDGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHK---YPN-VKISIDQVKPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSN--YSFIWAMCGENITYTDMDMVQMDQLK 4E2Q Chain:M ((89-258)) --------------------------------------------------------------------------DIERL-IFVVEGAVTLTN-SSSKKLTVDSYAYLPPNFHHSL-DCV---ESATLVVFERRYEYLGSHTTELIVGSTDKQPLL-ETP--G-EVFELRKLLP-M-SVAYDFNIHTMDFQPGE-FLNV-KEVHY-NQHGL--LLLE---GQGIYRLG--DNW--YPVQAGDVIWMAPFVPQWYAALGKTRSRYLLY--------------------

General information: TITO was launched using: RESULT: Template: 4E2Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 709 -18371 -25.91 -113.40 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain M : 0.63 3D Compatibility (PKB) : -25.91 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_4E2Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E2Q-query.scw PDB file : Tito_Scwrl_4E2Q.pdb: