Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MILVNIEGRLEKDDGTEEKKGIQKEVTIDRKANLKSHRLYWIGRRAQDVFCSALALIILSPVMLITALAIVIDDLGGSPIFAQNKVSR-------NGRLFKFYKFCSMCVDAEAKLDALLSQNEMDGPVFKIKNDPRITRVGKFI 4QXA Chain:B ((9-179)) ATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAVTIRLEEIVYLHCHQQV-DSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKG---KVATDYVFRIIYP--

General information: TITO was launched using: RESULT: Template: 4QXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 507 19368 38.20 146.72 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 38.20 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.034

(partial model without unconserved sides chains): PDB file : Tito_4QXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QXA-query.scw PDB file : Tito_Scwrl_4QXA.pdb: