Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGALAVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRKDGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMP-DGVPPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSALLLAFITPFLWFFGLHGANMV--DPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSFVNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMFIPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLVLSILIYLPFVKVATIQERRKEALDA- 4O6M Chain:A ((29-370)) ----------------------MRLAYVKNHEIYGEKLLGLTLRERIEKTLQRAGFDVRFFDELSLEE---------AEDYLIILEPVLILERDLLLEG-RKILVSDG-FTVGYFFG--GDFRTVFDGNLQSSIEKYLSLN---NLESYEIWAIKLSNDNLKTAEKLLLSSLIDGWIAREINRKVSLRISRLLADTSVT----------------------PNQITVFSFFLSLVGSALFLLNSYLTTLLAGVIIQLHSIIDG--CDGEIARLKFMES-----KYGAWLDGVLDRYSDFIIVFSITYVLSAS--------------------NPVYWIIGFLAAFASLMIAYTGDKFVAAYMRTYSPEGFAIPITRDFRLLIIFACSVVNLPSLALVIIALL----GNFEALRRIVALRSY

General information: TITO was launched using: RESULT: Template: 4O6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1551 -209174 -134.86 -641.64 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -134.86 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_4O6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6M-query.scw PDB file : Tito_Scwrl_4O6M.pdb: