Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFV-I-FGKMIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPTHVIIWGFVIGGVGRSILVGSLVTIISLF-FV----PLHVYSWFMVIITLLMT----AILFSLAGLLNGIFAQSYDDVSIVPTFVLQPLTYLGGVFYAI------------------------SMLPPFWQAVSKVNPIVYMIS----------GFRYGFLGTIDVPVATSMIVLVLFIVVL----YAVTWYLINKGRGLRS 5CR9 Chain:A ((15-309)) ----------HFPLTITNCGVDVTFDGPPERIILLESAPVATMRALGVLDSVVLRAGAFPPEYYDAETNAALRAIPSLGEELDS--SGHLQISEEVIIAQQPD---LVLGLPDGVTREGLEAVGINVLVQPTMCPGGVGATTFDDVYEQINTYGRLFDRQDRAAELVASLRQRVAAVEKAVEKRRSAAVLYPTIGGGVGYAYGNESMAHPQLESAGFTNVYADVDERVFEVTLEDVLEQDPDVLVLLHVDGDPDAVKDAVVNLPGADALTAVRNDDILVQLFNFTEPPTPLSVDGLERIHETFGA

General information: TITO was launched using: RESULT: Template: 5CR9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1113 -104096 -93.53 -431.93 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -93.53 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.157

(partial model without unconserved sides chains): PDB file : Tito_5CR9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CR9-query.scw PDB file : Tito_Scwrl_5CR9.pdb: