Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVSYLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAWGSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSAGDMIQDI--SKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRMQKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLIPYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTILLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE 3F6N Chain:A ((3-72)) ----------------------------------------------------------------------------------------------------------NLNQIQKEVSEILSDQKSMKADIKAILELLGSQN---PIKESLETVAAKIVNDLTKLINDCPCNKEILEALGT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3F6N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 14 147 10.50 2.16 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.39 3D Compatibility (PKB) : 10.50 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.39 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3F6N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F6N-query.scw PDB file : Tito_Scwrl_3F6N.pdb: