Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKNLTVIGSYSTREEALSVIERLRN--EGYERDDIVIYTTDEAASRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLY-----QYRGDISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQNTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEE---KLDVNTHD---VTTGEV--DIHKHVVNDTETVEVPVKRE-EIVIERKP---VTDQSSQGTDEKLE-DDTITIPIKEEQVDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS-------- 3P4G Chain:A ((23-323)) -HSFDHYIGSAFDASNNNVAVTGNVSATLNVLAGDDKVSIDGN-VEDVLVAANVAVLDMGTGNDQLYVAGD--VLGKID------------AGTGNDEIYIKGDVSAAVDAGTGNDEVYIGG--NLSGDLDAGTDNDNIQIGGDVNAALNAGTGNDNLIIG-----HDVSGIVNMGTDNDTVEVGRTINASGKVL-------LDTGDDSLLVSGDLFGEVDGGTGNDTIIIAGKVSGNIQGGTGNDIVRVQSQVWAEANISLGTGDDVLIVEHELHGTVAGNEGDDSIYLKF------------------YTKEQYNNNSDLRNRVANFE-------HIRVSDGVVKGSPADFADY

General information: TITO was launched using: RESULT: Template: 3P4G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1899 153779 80.98 563.29 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 80.98 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_3P4G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P4G-query.scw PDB file : Tito_Scwrl_3P4G.pdb: