Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQEVKSGKVLSPSTPWTQRPVPGIEVADEQQTLKATFTEPTIECPECHALVTRTAISFNAYVCPQCDEHLRMKARDRLNWFFDNV-VAELGQEFSAKDPLKFVDSKPYPDRMREAQTKTGETEALIAMQGNLNGVDMIACAFEFDFMAGSMGTVVGDRFVKAAELAIEKRQPLICFAASGGARMQEGMLSLMQMARTSAAIQKLKDTGLPYIVVLTHPVYGGVTASLAMLGDIHIAE-PKAMIGFAGKRVIEQTVRETLE-EPFQRAEYLLD-HGVVDQIVH-RHALRDTVSRLVSKLMNLP 2BZR Chain:F ((44-294)) -----------------------------------------------------------GEDAVEKVHAKGKLTARERIYALLDEDSFVELDALA-KHRSTNFN-----------LGEKRPLGDGVVTGYGTIDGRDVCIFSQDATVFGGSLGEVYGEKIVKVQELAIKTGRPLIGINDGAGARIQEGVVSLGLYSRIFRNNIL-ASGVIPQISLIMGAAAGGHVYS-PALTDFVIMVDQTSQMFITGPDVIKTVTGEEVTMEELGGAHTHMAKSGTAHYAASGEQDAFDYVRELLSYLPP--

General information: TITO was launched using: RESULT: Template: 2BZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1232 -62590 -50.80 -280.67 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain F : 0.67 3D Compatibility (PKB) : -50.80 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_2BZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BZR-query.scw PDB file : Tito_Scwrl_2BZR.pdb: