Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLRSAMHASDVDDAGRALQSTQRSIVSISRLTNEMSEVRAMSEGSFG-VVKCYRHDFDKLEYAVKQTKRPICGESNLQQQLQEIYALSSF-PHRHIVRYFDGWVEDRAVFVRLEKLDDCMASLPPPVSESV----------LTAMLHQTSTALYELHSHDVVHMDVKPENIL--RRQLDADTF--IFKLCDFGLARPLNGKDSVTGEHFLGLNDDDGDRRYMSPELLKNLHDVVGPPADMYALGKSCEAMMTATEDPSNTSARHL----------ESYSPAFIALIESMLCEDPARRPSAFQVVQATLPERLLSDGGLLELQRRIDAIRCEISDLDASDNDVMSSPRT 2Z2W Chain:A ((3-278)) -----------------------------SRYTTEFHELEKIGSGEFGSVFKCVKR-LDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNG--GSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFIS---------KVMFKIGDLGHVTRISSPQV-----------EEGDSRFLANEVLQENYTHL-PKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSV----------------------------------------

General information: TITO was launched using: RESULT: Template: 2Z2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1092 34306 31.42 149.16 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 31.42 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_2Z2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z2W-query.scw PDB file : Tito_Scwrl_2Z2W.pdb: