Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNYFSLCPEPFHGLISSYGIGSTSSTHPSGVAVKGQEYSAVKLVGEGGYSFVYEGYNNASGQRVALKRYVFSETQQQQGAVEEMSIYRDVCPNDYIVTYLDSEVVYRHGVPLPEMWVVMEFCDGPSLQEYINNRLRSPQPFSVREVFEIVDNIVHAIGHLHSQSPPVSHWDIKPENFLFTDTGRLKLCDFGSATRQFYAPTSAEEVSAAESELGSRMTLLYRPPESLDLWSKDRVDTKADIWALGVIIYVLVFREMPFDANPMEVMAA---VPKRYKGKTQEGCPEEFRALMDIVRTKMLTKKPADRADIFAISEELEGITHLPPLSRPRPGFQSAQRPRFA 5TE0 Chain:A ((19-283)) ------------------------------------QQVTVDEVLAEGGFAIVFL-VRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNV----VWEVLILMDFCRGGQVVNLMNQRLQTG--FTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQT-EGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHC---------LIRY-MLEPDPDKRPDIYQVS----------------------------

General information: TITO was launched using: RESULT: Template: 5TE0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1293 -146332 -113.17 -567.18 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -113.17 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_5TE0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TE0-query.scw PDB file : Tito_Scwrl_5TE0.pdb: