Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVSTLQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLLGEHLEAKHQERAQYYSKAPTEL-IYEDAELVH-DKTTIDLFLAFSNILAKKKEEFAQNHTTILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELILVQEESFGDIYLMEKKEESQVPQS 3W6J Chain:A ((8-168)) -NVKIEAFEGPLDLLLHLINRYEIDIYDIPVAQITEQYMAYIHAMQELELDIASEYLVMAATLLAIKSKMLLPP---------DPREELTQRLLEYKKFKEAARELKRREEERALLFTKPPSDLSAYA------LDVNVYDMLGALSKLLRRKK------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W6J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 300 -59001 -196.67 -433.83 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -196.67 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_3W6J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W6J-query.scw PDB file : Tito_Scwrl_3W6J.pdb: