Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFEDQSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIGVTIALVNTSQVGKVLAHSINLVKPIAVIAGEEVRAAIDEIRQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQFPKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWTLAYGTYGHILNLG-PDDVMYVTLPLYHATGVVVCWCGVIAGSATLAIRRKYS---TSAFWKDVQKFNASAIGYVGELCRYLMDAPVTELDRNHRVTKMIGNGMRPNIWD--KFKQRFGVKEVLELYASSEGNVGFSNIFNFDNTVGFSPTPYAIIQFDKE-KNEPIRDKNGWCQKVKAGEVGLLIGKITSRSPFDGYTD-PEKNKSVIWKDVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWKGENVSTTEVENMVCEYDKIAEAVVYGVEIPNTNGRAGMAAITLADGEELNDADLTEMVTVFKKCLPTYAVP--------VFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCDITTEIFDNIQAYKYRF 2VSQ Chain:A ((470-995)) ----------------------------------------------------------------------------FKEAVNANPDAPALTYSGQTLSYRELDEEANRIARRLQKHGAGKGSVVALYTKRSLELVIGILGVLKAGAAYLPVDPKLPEDRISYMLADSAAACLLTHQEMK------EQAAELPYTGTTLFIDDQTRFEEQAS-----------------DPAT--AIDPNDPAYIMYTSGTTGKPKGNITTHANIQ---GLVKHVDYMAFSDQDTFLSVSNYAFDAFTFDFYASMLNAARLIIADEHTLLDTERLTDLILQENVNVMFATTALFNLLTDAGE---DWMKGLRCILFGGERASVPHVRKALRIMGPGKLINCYGPTEGTV-----FATAHVVHDLPDSISSLPIGKPISNASVYILNEQSQLQPFGAVGELC--ISGMGVSKGYVNRADLTKEKFIENPFKPGETLYRTGDLARWLPDGTIEYAGRIDDQVKIRGHRIELEEIEKQLQEYPGVKDAVV--VADRHESGDASINAY-LVNRTQLSAEDVKAHL---KKQLPAYMVPQTFTFLDELPLTTNGKVNKRLLPKPDQDQLAEEWIGPRNEMEETIA--------------------------

General information: TITO was launched using: RESULT: Template: 2VSQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2718 -42439 -15.61 -86.08 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -15.61 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_2VSQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VSQ-query.scw PDB file : Tito_Scwrl_2VSQ.pdb: