Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIRITGTGLFHPTEIISNEELADSLNAYVEQYNQENAEKIAAGELEELRGSSAEFIEKASGIKRRYVIEKSGILDPTRLRPRLSERSNDELSIQAEWGVIAAKQAMENAGVTAEDIDVVILACSNMQRAYPAVAIEIQSALGIQG-YAYDMNVACSAATFGLKQAADAIRSG-ARRVLLVNVEITSGHLDYRNRDCHFIFGDVATASIIEETTTKTGFEILDIHLFTQFSNN---IRNNFGFLNRSEDAVVDDKLFRQDGRKVFKDVCPLVAKIINAQLEKMQLTANDIKRFWLHQANAN-MNELILKYVAGKDADLSRTPIILDEFANTSSAGVIIALHRTGHE--VDDGEYGVISSFGAGYSVGSIVVQKHVA 2EBD Chain:A ((1-308)) MGTKIIGTGVYLPKNVLTN------------------------FDLEKIVDTSDEWITTRTGIKERRIAK---------------EETITYMATQA------AKEALREANLSPEELDLIILATLTPQKRFPSTACLVQAQLKAKGVYAFDISAACSGFIYALDIADSFIKSGKAKNVLVIGAEKLSEAVDWEDRSTCVLFGDGAGAVVVTRSEDKS--DILATRMYAEGSLEELLHADNCGYI-------------RMKGRELFKVAVRSMEEVCREVLEKAGVKPEEVSLVIPHQANVRIINALAEKLNIPKE----KVFVNIQKYGNTSAASIPIALHEAIKEGKVKRGDLILMTAMGGGLTWGAVLLR----

General information: TITO was launched using: RESULT: Template: 2EBD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1857 -90907 -48.95 -303.02 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -48.95 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_2EBD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EBD-query.scw PDB file : Tito_Scwrl_2EBD.pdb: