Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKWLQIHITVDQEQVEFTETLLMSLGAVSVTLDDAEDQALLEPLPGETPLWNKVIVTGIYQQDEQDPIDVDTLEAFLKAQLPDVPMRHEELEDQVWERAWMDYYEPIQIGEKFWIVPEWLEPPEADATNIKLDPGLAFGTGNHASTFLCLQWLGKTDVKNKIVIDYGCGSGILGVAALLLGAKKVYATDIDPQAVLATKQNAELNGVLDRLYVGLPEEFDQEFKPQQADVLVANILAGPLMALAPEFAKLLKSDGDFALAGVIEEQVVDVSGVYSEFFDILDVEKREENWCRISGKRKTTN 1UFK Chain:A ((52-224)) ----------------------------------------------------------------------------------------EEVGDEDWLEAWRRDLKP-ALAPPFVVLAPW-HTWEGAEIPLVIEP------GHHETTRLALKALARHLRPGDKVLDLGTGSGVLAIAAEKLGGKAL-GVDIDPMVLPQAEANAKRNGVRPRFLEG-SLEAALPFGP--FDLLVANLYAELHAALAPRYREALVPGGRALLTGILKDRA----------------------------------

General information: TITO was launched using: RESULT: Template: 1UFK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 827 -68872 -83.28 -412.41 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -83.28 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1UFK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UFK-query.scw PDB file : Tito_Scwrl_1UFK.pdb: