Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKTLQFGITNFEIDFKDLVMNTMIKNKRFENDVEFRGADIKDSRAVVILLHGRRQNADTIYELAE--KIGFTDITYILPLAPEL-----------TWYPRGFMRDLEDNQPYLSVALE------RIHYIITDLKNQNIKPENIFLMGFSQGACIVSQYLWENPQKLGGVIAFTGGL-----FGPELTYTSHEGLPINGTQLIFTGGKEDSWVPEQRVRDTAALFEKLGGNVTTKIYSGRDHLVSDEEVE-----LAKTLLNI 4F21 Chain:A ((34-244)) -----------------------------------------KQARFCVIWLH--GADGHDFVDIVNYFDVSLDEIRFIFPHADIIPVTINMGMQMRAWYD---IKSL---SLNRVVDVEINSSIAKVNKLIDSQVNQ-IASENIILAGFSQGGIIATYTAITSQRKLGGIMALSTYLPAWDNFKGKIT-SINKGLPI-----LVCHGTDDQVLPEVLGHDLSDKLKVSGFANEYKHYVGMQHSVCMEEIKDISNFIAKTF---

General information: TITO was launched using: RESULT: Template: 4F21.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 866 -37790 -43.64 -215.94 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -43.64 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4F21.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F21-query.scw PDB file : Tito_Scwrl_4F21.pdb: