Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAALMCFSSLAQAYQYEQTARLIN---ERLSYMKDVAGYKAEQHLPIEDLTQEKKVLDQSLSEADAFGLNSETVKPFIVAQMDVAKAIQYRYRADWLSSPETN-WKPQDLSEVRVKISALNTELLKNIAYELKKNNNKAPHGCSYMWPVQHPQLKEADKRALCVTLNKIKLKQ 2F6L Chain:A ((38-148)) -----------------QLAELVDAAAERLEVADPVAAFKWRAQLPIEDSGRVEQQLAKLGEDARSQHIDPDYVTRVFDDQIRATEAIEYSRFSDWKLNPASAPPEPPDLSASRSAIDSLNNRMLSQI---------------------------------------------

General information: TITO was launched using: RESULT: Template: 2F6L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 270 7846 29.06 73.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 29.06 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_2F6L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F6L-query.scw PDB file : Tito_Scwrl_2F6L.pdb: