Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIFSTNTCPVPNDLESVIHHKDEVPAELCGMSEQQVQKIWKSIESLYKTGNYPLITFCLRRQGKILLNRSIGYAQGNSPAGLQENALIATPDTPVCLFSASKMITAMLIHLLDEKGEINLLDPVSYYIPEYGVNGKRRATIFHLLSHRGGIPYVDGDVTPELLFDKDEILRRLYAARPVSPAGNHLAYHAVTAGYILGEIIERVTGQDLRKFVHETIEKPMDMPYFNYGL--KPEYRAEVALNCATGLHPRLGTDHYLNHVLGGGLQLAVDVTNDSRFMDTICPAGNIYTSAEQAGRFFEMLLSGGSYQGKQ----ILSEKTIFRATLPTTGVNIDRTLLIPMRYALGPMLGSN--PVGLFGPMTGQAFGHLGFSNILCWADPERDISVSLLTTGKSVVGTHLPALANLLYQISIQCPRIPRDQRRSLFGSDSHETDLV 3ZYT Chain:A ((51-334)) -------------------------------------------------------------------------------------------PDSVTGVFSCSKGMAGLVMALLVQDGELDLEAEVVKYWPEFGVEGKSSITVAQLLSHRAGLLGVEGGLTLHEVNNSELAAAKLAELPPLWKPGTAFGYHALTIGIFMEELCRRITGSTLQEVFEQRI-RAVTGANFYLGLPESEESRFAQFRWAADPSWPWVDPASHFGLAANAAVGDILDLPNIREVRAAGLSSAAGVASAEGMARIYAAALTG--LEGKSAMPPLLTEETI-RTVSAEQVFGIDRVFGETGCFGTVFMKSHTRMPFGSY-----RAFGHDGASASLGFADP--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1527 -121030 -79.26 -438.51 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -79.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_3ZYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZYT-query.scw PDB file : Tito_Scwrl_3ZYT.pdb: