Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTKKDLLQQRMLIDQLRTQNSLSPQNAAKLGQSIASSWERSASAAIPKERFAAP----------LLEKKSASQNALDLALSQCADDLRHIAEQSSMVIAVGDIGSTIIWTASSAQMQSAAERVHFVQGGQWREEFVGTNALALSLKTQQSSCVFSNEHYMESIHDWVCYAAPIIDPYSKQTLGVVDLSTTWKNHNTLGILAAERCASIIQSALLEQQRQQLHIRAFSTPQVKFNGKSLLLTPRQIEILTILALCPHGLTLENLYQALYGERKVSMGTLKAEMSQLRDILGGLLGSRPYRLLVHVEADFLQAEQALDAGYVASALQLYAGVFLAKTESPFLCAWRDCLESRLSDAIFKAQETDLLLKHLAHFPEAIDAVERLMELLPSEHPAHQLLVKYIDSPKLS 4LRY Chain:C ((16-164)) ----------------------------------IATSWER-CNKLMKRETWNVPHQAQGVTFASIYRRKKA---MLTLGQAALEDAWEYMAPRECALFIL-DETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQVLWNWAFCATPLFDSKGRLT-GTIALA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LRY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 577 -24886 -43.13 -179.04 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.59 3D Compatibility (PKB) : -43.13 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.405

(partial model without unconserved sides chains): PDB file : Tito_4LRY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LRY-query.scw PDB file : Tito_Scwrl_4LRY.pdb: