Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSPSNPVLSHHISSQFNEDLQDVNTKFMTMGGLVEQQVANAIHSLLDTDANLAIDVQFKDNAVNQYERDIDEGLTLILARRHPAAIDLRMVIAMSKANTDLERIGDEAAKIARIAQNLCEEGGSPRGYMETRHIGNQVRVMIHDALDAFARLDADQALRVLLADVDIDREYQSATRTLMTYMIEDPRHIARVINVMWVLRSLERIGDHARNIAEQVIYMAKGFDARHTKIEEIEAKVHEK 1T8B Chain:A ((12-214)) ------------------ELEETKEQVIKMAKLVQEAIDKATEALNKQNVELAEEVIKGDDTIDLLEVDIERRCIRMIALYQPEAGDLRMIMGIYKIVSDLERMGDEAENIAERAILLAEEP-PLKPYVNINFMSEIVKEMVNDSVISFIQQDTLLAKKVIEKDDTVDELYHQLERELMTYVLEDPRNIKRAMHLSFVARHYERIADHAENVAEAAIYLSEG------------------

General information: TITO was launched using: RESULT: Template: 1T8B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 916 -97935 -106.92 -482.44 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -106.92 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_1T8B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T8B-query.scw PDB file : Tito_Scwrl_1T8B.pdb: