Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNATLNYQPHWVREDFVDFVLQKINATWAWKRVLAEVTAVQSLHTDMV--LIKLKP----NRNFNFDQVRAGESILLTLLIDGVYQQRSYSIIEVTT-QGEIALGIKV--QGLVSRAAQ-LLHVGECVEISQPQGDFTLHQ-GQQPAILIASGSGVTAIYSLLQQALKQQLEQIHVIYFN---RAEIFH-AELKALAEQYPQLQ-----YHFFNTTEQKQHLTESLLQKLVPDFEQTAT----YVCGHHGMMQQANEIYTQKG-AQSQLHQEYFQPLQVTGTHAAQPVIFRRAQQEFLAETNLLGSAEQAGLRPQHGCRMGVCNKCSCTKVSGVTQNLLTGEIEDQPNRPIKLCVSQALSPVTIDL 1TVC Chain:A ((13-246)) ----------------------------------AEVVGLNWVSSNTVQFLLQKRPDECGNRGVKFEPGQ-----FMDLTIPGTDVSRSYSPANLPNPEGRLEFLIRVLPEGRFSDYLRNDARVGQVLSVKGPLGVFGLKERGMAPRYFVAGGTGLAPVVSMVRQMQEWTAPNETRIYFGVNTEPELFYIDELKSLERSMRNLTVKACVWHPSGDWEGEQG---SPIDALREDLESSDANPDIYLCGPPGMIDAACELVRSRGIPGEQVFFEKFLP------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TVC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1094 11245 10.28 53.80 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 10.28 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_1TVC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TVC-query.scw PDB file : Tito_Scwrl_1TVC.pdb: