Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIKKRGLAKGRGLDALLGSIQKEKLQLEAQALDHGQLKQIDVKLLKRGEYQPRRFIHEHDLQELASSIEKHGVMQPIVIRPVDDEAHPYEIIAGERRWRAAQIAGLTEIPAIVRDLNDQVAIALALIENIQRQDLNPIDQALALQRFHDEFGLSHQEIADTVGKARTTVSNLLRLLSLADDIKEFMQQGQLDMGHARAILTLKGKEQLEVAKIVIEKGLSVRQTEQLVRDWNEPKQEKEKAPVAPDIEQLTQKLSERFGANVKIDHNQKGKGKLVIHYHSLDELDGILNICLPD 4UMK Chain:A ((55-224)) -----------------------------------------------------------DSLEELAQSIKEHGLLQPVL---VVSENGRYHLIAGERRLRASKLAKMPTIKAIVVDIEQEKMREVALIENIQREDLNPLELARSYKELLESYQMTQEELSKIVKKSRAHVANIMRLLTLSSKVQNALLEEKITSGHAKVLVGLDGEKQELILNSIIGQKLSVRQTEDLARDF---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 560 -58079 -103.71 -341.64 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -103.71 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_4UMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UMK-query.scw PDB file : Tito_Scwrl_4UMK.pdb: