Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELLTLARPYAKAAFAYASEQGATDNWSNALQVLSAAVQDEAFSAYLNR---PEHTPAEQVKLFAKVLGEDQSQAVSNFLTLLADNDRLVLLPEIAAEYEQLKSQNNNNVDVVIESAFPLTAEQEQLLKSALEKRFNSTVTVSVEVKPELIAGVVIRAGDQVIDDSALNKLEKMRTRLLA 5T4P Chain:L ((3-162)) --EFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQMAELLSGALAPE-TLAES---FIAVAGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAVSEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNAKIDKSVMAGVIIRAGDMVIDG---------------

General information: TITO was launched using: RESULT: Template: 5T4P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 594 -16303 -27.45 -103.84 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain L : 0.75 3D Compatibility (PKB) : -27.45 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_5T4P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T4P-query.scw PDB file : Tito_Scwrl_5T4P.pdb: