Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVALTPELLTQLTAIVGENRIKTD---------ADSLENWGKDHTKHFNPNPSVIVFPSTTEQVQEVVKLANQFNIAITPSGGRTGLSAGAVATKGE---IVISMDKMNQILEFFPADRMVRVQAGVVTEQLQNYAEEQGMYYPVDFASAGSSQIGGNIGTNAGGIKVIKYGMTRNWVLGLTVVTGKGDILRLNKGMIKNATGYALQHLFIGGEGTLGLVTEAEIKLERQPQNLQVLVLGVPDFDAVMPVLHAFQK-----------DIDLTAFEF--------FGELTMQKVLDRGHVQRPFETQCPFYVLLEFEAPYEPIIDKAMEIFE----------------HCMEQGWVLDGVMSQSLDQVESLWRLREDISESIAPFIPYKNDISVLITHVPAFIREIDAIVQENYPYFEICWFGHIGDGNLHLNILKPENLTKDEFFAKCQVVNKYVFDTVKKYDGSISAEHGVGMTKKPYLEYSRSAEEIEYMKALKKVFDPKGIMNPGKLFDL 2UUU Chain:A ((87-583)) ---PPKQYPEFVRELKAFFLPDQLKDDKLARITHTFGKSLRDLIRVRIGQVKNAPDLIVLPHSHEEVERLVQLAHKYNVVIIPMGGGSNIVGAIEPVSNERFTVSIDMRRMNKVLWVDRREMTACIQVGIMGPELEKQLHKQGVSLGHDPDSFEFSTLGGWLATCSSGHQSDKYGDIEDMAVSFRTVTPTG-TLELR--------GINYKHIILGSEGTLGIITEAVMKVHAVPQAVEYYGFLFPTFAHAVSALQQIRSSEVIPTMIRVYDPEETQLSFAWKPSKGAVSEFTSAMVKKYLHYIRSFDFKNVCLSIIGFEGPKKVVDFHRTSVFDILSKNAAFGLGSAPGKTWAEKRYDLPYIRDFLLDH--NMW---VDVAETT---VSYANLQTLWKDAKQTFVKHFK---DQGIPAWICAHISHTYTNGVCLYFIFASKQNENKDMAQYIEAKKLMTDIIFKYGGSLS------------------RGWINVYRSLKETIDPKDICNPRKL---

General information: TITO was launched using: RESULT: Template: 2UUU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2198 3251 1.48 7.65 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 1.48 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_2UUU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2UUU-query.scw PDB file : Tito_Scwrl_2UUU.pdb: