Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPAYKAPLHDIRFLMNEVLDYPAHYKTLSNGESADPDTVDMILEGAADYCENVLSPLNQSGDEEGCHFDNGEVKTPKGFKEAYDQFVMGGWQGLSYPEEFGGQGLP-MSLNLIKSEMMGTANWSFTMYPGLSSGCMNTILQFGTDEQKQTYMPKLVEGTWSGTMCLTEPQCGTDLGQVKTKAEPQADGTYKISGTKIFISAGEHDLTENIIHIVLARLPDAPAGTRGISLFIVPKFIPTAEGGVGERNTVSCGSIEHKMGIRASATAVLNFDNA---TGYLIGEVNKGLHAMFTFMNTARIGTAVQGIAHAELSFQGALPYAKERMSMRALSGKKDPDKVADAIIHHADVRRMLLTQKAIAEGGRSMIYYAAQLADKMTDALTRGDQAAFEDYDDKLGFYTPILKGFLTELGLEAANHGMQVYGGHGYIKEWGMEQIVRDARISTLYEGTTGVQALDLIGRKVLLSSKGKVVREYTAEILKFCAAHARNKYLRRFAWDLTKLCAQWNTLTVRIMLAARKDRDIVSSASVDFLMFSGYVMMAYFWAQQAVVASEKLEAGNGQETPEFYKAKIKVADFYFDRLLPRAQGHAESMVTTSRTLTSLPAEHFSFDY 4L1F Chain:A ((11-380)) ----------------------------------------MIKDMAAEFAEKFLAPTVEERDK--AHIWD---------RKLIDKMGEAGFCGICFPEEYGGMGLDVLSYILAVEELSKVDDGTGITLSANVSLCATPIYMFGTEEQKQKYLAPIAEGTHVGAFGLTEPSAGTDASAQQTTAVLKGD-KYILNGSKIFITNGK----EADTYVVFA-MTDKSQGVHGISAFILEKGMP------GFR----FGKIEDKMGGHTSITAELIFEDCEVPKENLLGKEGEGFKIAMETLDGGRIGVAAQALGIAEGALAAAVKYSKER------------EQFGRSISKFQALQFMMADMATKIEAARYLVYHAAMLKN---EGKPYSEAAA-------------MAKCFASDVAMEVTTDAVQIFGGYGYTVDYPAERYMRNAKITQIYEGTN--QVMRIVTSRALLRD------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4L1F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2234 -56839 -25.44 -155.30 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -25.44 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_4L1F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L1F-query.scw PDB file : Tito_Scwrl_4L1F.pdb: