Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYRHHFHAGNFADVMKHVLLLQLLNRLNAKDKPYRYIDTHGGAGKYDLSQAPAQKSGEFLTGIHRLVQLSGMEKRQAPEAIQQYLKLVEELRAQEGKGSYPGSPWFALQGMREIDKATIFEMQRDVFQQLRHNIH-DKRAGLHERDAYEGLLAVIPPKEKRGLVMIDPPYELERKDFPQLVELLQSAYKKWPTGVFAVWYPIKDRAMIERFEKKMFKTGIRRQLICEICVWPDDTPVGLNGCGLLVINPPWQFSEQADQALQWLFPHLRMQETG-GHAAVRWLVGE 4BLV Chain:A ((2-280)) LSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAGAGRYQLGSEHAERTGEYLEGIARIWQQDDL-----PAELEAYINVVKHFNRSGQLRYYPGSPLIARLLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGLILIDPPYEM-KTDYQAVVSGIAEGYKRFATGIYALWYPVVLRQQIKRMIHDLEATGIRKILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWL--HSKLVPAGTGHATVSWIVPE

General information: TITO was launched using: RESULT: Template: 4BLV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1565 -93353 -59.65 -337.01 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -59.65 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_4BLV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BLV-query.scw PDB file : Tito_Scwrl_4BLV.pdb: