Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSLSLLHPYPFEKLNQLFKDTTPANLPLIPLSIGEPKHPAPEFVKQAIIDNFNHLSTYPNSKGLPELRQSIADWLTKRFKLNSISAENHILPVSGTREGIFSFVQTLINREDAPYVVMPNPFYQIYEGAALLAGAKPYFINCTEENGYLGDFDAVPAEVWEKTALLFVCTPGNPTGTVLSKEQFKKLIALSDQYNFVIASDECYSELWFDQAPTGLLEVCAELGRGDYKNCIVFHSLSKRSNLPGMRSGFIAGDAALLKPYLQYRTYHGAAMPVQHQLASIAAWNDESHVEENRKQYRAKFDLFQSELGHLLPLQKPDAGFYYWLKVDNDETFAKMLMEKAHIKVLPGRYLSRDTEQGNPGANHVRMALVADLAQCEEVVKRLKAIL 3EZS Chain:A ((4-363)) -------FEPYPFERLRALLKEITPKKRGL-DLGIGEPQFETPKFIQDALKNHTHSLNIYPKSAFEESLRAAQRGFFKRRFKIEL--KENELISTLGSREVLFNFPSFVLFDYQNPTIAYPNPFYQIYEGAAKFIKAKSLLMPLTKENDFTPSLNE---KELQEVDLVILNSPNNPTGRTLSLEELISWVKLALKHDFILINDECYSEIYENTPPPSLLEACMLAGNEAFKNVLVIHSLSKRSSAPGLRSGFIAGDSRLLEKYKAFRAYLGYTSANAIQKASEAAWLDDRHAEFFRNIYANNLKLARKIFKNTLIY---PYSFYVYLPVQNGENFAKTLYQNEGIITLPALYLGRN----RIGADYVRLALVYDTPLLEK---------

General information: TITO was launched using: RESULT: Template: 3EZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2018 -212707 -105.40 -590.85 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -105.40 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_3EZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZS-query.scw PDB file : Tito_Scwrl_3EZS.pdb: