Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKANQEFLRQAIELAYNNIEKG-GRPFGAVIVKDGKVIASGVNQILTTNDPTAHAELLAIRAASQVLGTANLEGCSVFASGHPCPMCMAAMRLAGIKTVNYAYSNEDGAPFGLSTAEIYADLVKPFAEQSMKIEYVPVRFEDRTDLYVFWKNYQAKQSGLK 1TIY Chain:A ((3-157)) ---HETFLKRAVTLACEGVNAGIGGPFGAVIVKDGAIIAEGQNNVTTSNDPTAHAEVTAIRKACKVLGAYQLDDCILYTSCEPCPMCLGAIYWARPKAVFYAAEHTDAAEAGFDDSFIYKEIDKPAEERT--IPFYQVTLTEHLSPFQAWRNFANKKEYL-

General information: TITO was launched using: RESULT: Template: 1TIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 641 -29294 -45.70 -190.22 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -45.70 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_1TIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TIY-query.scw PDB file : Tito_Scwrl_1TIY.pdb: