Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKPYYEFFCPVKVIAGNAALEHIPFELATLGAKRPLIITDKGVR--ANGLLNPIEAAF--STTDAVIGHVFDDVPPDSSLEVVRLAAEAYRTNKCDAIIAVGGGSVIDTSKATNILVSEGGDDLLQYSGAHNLPKPLKPFFVIPTTSGTGSEVTMVAVVSDTQKNVKLAFASYYLMPHAAILDPRMTLTLPPHLTAMTGMDALTHCVEAYTCMAANPLSDAYASAGIKKISENLFNVLENPNDSQGRLELAQASTMAGIAFSNSMVGLVHSLGHALGAVAHLPHGLCMNLFLPYVLEYNKQINGQKIGELLLPLAGADIYAQTPATQRADRAIATILAMRDRLFSLTKLPRTLRETGKVTEAQLDEVAEKALNDGSIIYNPKEASLKDLREILQKAW 4FR2 Chain:A ((16-398)) ----AYDFLMPSVNFFGPGVISKIGERAKMLGMKKPVIVTDKFLENLKNGAVAQTLASLKKSGVDYV---VYNGVEPNPKIHNIKEVKTLYEKEDADSIITVGGGSAHDTGKGAGIIMTNG-DDITKLAGIETLKNPLPPLIAVNTTAGTGSELTRHAVITNEETHLKFVVVSWRNIPLVSFNDPTLMLDIPKGLTAATGMDAFVQAVEPYVSVDHNPITDSQCIQAIKLIESSLREAVANGHNLQARTKMVEAEMLAGMAFNNANLGYVHAMAHQLGGQYDAPHGVCCALLLPYAEEYNLIADPERFAELARIMG-----ENTDGLSTRDAAELSIKAMK-QLSEDVGIPHSIKDIGAKPE-DFDLMAENALKDGNAFSNPRKGTKEDIVKIFQEAY

General information: TITO was launched using: RESULT: Template: 4FR2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2323 -44359 -19.10 -117.04 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -19.10 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_4FR2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FR2-query.scw PDB file : Tito_Scwrl_4FR2.pdb: