Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSATGLTGSGSRDWFIQRVSAVVLAAYTVVLFGWILCKGGFDYQQWAGFMMTLPMKIFSLLAILSLIAHAWIGMWQVFTDYVTTRQMGPSAKGLRLVLTTAVIIAVFVYAIWGIQIFWAN 1NEN Chain:D ((3-84)) -SNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELTYEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYV--------------------------------------

General information: TITO was launched using: RESULT: Template: 1NEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 159 -26508 -166.72 -323.27 target 2D structure prediction score : 0.90 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -166.72 2D Compatibility (Sec. Struct. Predict.) : 0.90 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.251

(partial model without unconserved sides chains): PDB file : Tito_1NEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NEN-query.scw PDB file : Tito_Scwrl_1NEN.pdb: