Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAIANNHVVSFHYKLTNAEGETLDQSQ-GEPLAYLHGAGNIIPGLENALTGKTVGDKFTVNVPAAEGYGEYNPDLVQEVPAQMFQGVDNIQPGMQFQAQTDDGVQIVTVKAVEGDNVIVDANFPLAGQDLTFEVEIVEIREASQEELDHGHVHGAGGHHH 2KFW Chain:A ((3-147)) ---VAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEEL-------------

General information: TITO was launched using: RESULT: Template: 2KFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 -2160 -3.58 -15.00 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -3.58 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_2KFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KFW-query.scw PDB file : Tito_Scwrl_2KFW.pdb: