Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFERGIGFFALIFSILVIGAFIALSIYLIRLDNIIREKFEGQRWDIPAKVFARPLEIYNNAPITQANFTQELKLLGYKTSSNYDKSGTYVAQGSNMYVHTRGFDYGDSVEPEQVLELSFANDQVVEVRSTKPSST-GVARLEPLLIGGIYPQHNEDRVLIKLNSVPKPLIEALISTEDRNFYHHHGISIRGTARALVSNVTGGRRQGGSTLTQQLVKNFYLTPERTLKRKVNEALMALLIELHYSKDEILEAYLNEVNLGQNGNYSINGYGLAAQFYFGLPLRELNVAQQAYLVGLVQGPSLYNPWKNPEGAKNRRDTVLNNMRVMGYLTQAEYETEIARPLNVLSKPSLGPAKFPDFLDIVRRQLRTEYQESDLTNQGLRIFTTLDPIAQTQVQNAFKASVERL-ANSNPARLKNLQGAVLIAHPENGELIAAVGSTQ-DFTGFNRALDAKRQVGSLLKPVIYLSAIESGR-YNWASQIEDAPISVPVDGGKSWTPKN----YSGGGHGIVSLSEALANSYNLSAVRLGQEFGLSTFTNNLRKFGV-ESTIPAYPSIFLGAVNMSPMEVLGIYENFATGGFKYPTRAIRSVVDANGHLLDRYGLNVQETIDPSVGYIMNYGLQQVMSSGTGRAAYNSLSPALKLAGKSGTTNDTRDSWFAGYSGNHVAVVWLGLDDNKVTGLTGSSGALPVWINVMKQLRQTPVNLRQPDSVQWQWIDHASGDLSAQACDGAMYIPMLAHTVPHRATPCGAPYYQVDPTYTPQSDNTIPEPEDDNTDSYIRESENQMEQDLSNNTRIISSGSYNN 5HL9 Chain:A ((11-725)) -----------LLLKLAIVFAVL-IAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMIS--NGEQRLFVPRSGFPDLLVDTLLATEDRHFY---------------------------TLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRG--ISPQ-PAFMQLVRQELQAKL---VKDLSGVKIFTTFDSVAQDAAE---KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGR--VMLVDALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR-QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDY-DGNF---VCSGGMRI----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5HL9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3683 -3908 -1.06 -5.82 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -1.06 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_5HL9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HL9-query.scw PDB file : Tito_Scwrl_5HL9.pdb: