Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRDRIMQITIRGHHLAITPAIEENIKAKFNQLTKHLDQVNSMQIKLTKDHQIDKRSHKGSSNHVAEAIVRLPGIELFAQATADDMYTSIKKLTEKLKKQLLKYRKMQCTYSQVAVSI 2RQL Chain:A ((1-89)) -----MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVL----KVEKVT------HTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHK-------------

General information: TITO was launched using: RESULT: Template: 2RQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 368 -47043 -127.83 -528.57 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -127.83 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.660

(partial model without unconserved sides chains): PDB file : Tito_2RQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RQL-query.scw PDB file : Tito_Scwrl_2RQL.pdb: