Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVETAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVHQDILKTDLSTAVRETFHEELPLK--VVANLPYYITTPIMMHFLESDLVVDELVVMMQKEVADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAVEVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGETLSLQEFAALSNAMSENMQ 3FUW Chain:A ((6-267)) --------SPQSVRALLERHGLFADKRFGQNFLVSEAHLRRIVEAAR-PFTGPVFEVGPGLGALTRALLEAGAEVTAIEKDLRLRPVLEETLSGLP-VRLVFQDALLYP---------WEEVPQGSLLVANLPYHIATPLVTRLLKTGRFA-RLVFLVQKEVAERMTARPKTPAYGVLTLRVAHHAVAERLFDLPPGAFFPPPKVWSSLVRLT-----PTGALDDPGLFRLVEAAFGKRRKTLLNALAAA----GYPKARVEEALRALGLPPRVRAEELDLEAFRRLREGL-----

General information: TITO was launched using: RESULT: Template: 3FUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1353 -44661 -33.01 -171.77 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -33.01 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_3FUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FUW-query.scw PDB file : Tito_Scwrl_3FUW.pdb: