Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNL-QAIIPSVKSAYNGQAVFGNEIIETFTQMM--KNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ 5HEV Chain:A ((1-206)) MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAMALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYLLKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVL----HEELTNRENEILMLISEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKH--

General information: TITO was launched using: RESULT: Template: 5HEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 913 -70189 -76.88 -345.76 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -76.88 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.698

(partial model without unconserved sides chains): PDB file : Tito_5HEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HEV-query.scw PDB file : Tito_Scwrl_5HEV.pdb: