Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNIATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQNEYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFFNKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDKMNQ 4J91 Chain:A ((8-214)) ---QFAVIGLGRFGGSICKELHRMGHEVLAVDINEEKVNAYASYATHAVIANATEENELLSLGIRNFEYVIVAIGANIQASTLTTLLLKELDIPNIWVKAQNYYHHKVLEKIGADRIIHPEKDMGVKIAQSLSDENVLNYIDLS----IVEL-L----LDSKSIIDLNVRA--GCTILAIKH-HGDICLSPAPEDIIREQDCLVIMGHKKDI---------

General information: TITO was launched using: RESULT: Template: 4J91.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 885 -167107 -188.82 -848.26 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -188.82 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_4J91.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J91-query.scw PDB file : Tito_Scwrl_4J91.pdb: