Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGT--GQATKASAGIISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVLAEK--RKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLE-HLGQRLAQKQMEQIQAKV-----TLHKKSD--SWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQKGQLLAFQTSYKMSQDWPV--AMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKGLTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLTTGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS 1RYI Chain:A ((35-359)) ------------------YLAKENKNTALFESGTMGGRTTSAAAGMLGAHAECEERDAFFDFAMHSQRLYKG------LGEELYALSGVDIRQHNGGMFKLAFSEEDVLQLRQMDDLDSVSWYSKEEVLEK----EPYASGDIFGASFIQDDVHVEPYFVCKAYVKAAKMLGAEIFEHTPVLHVERDGEALFIKTPSGDVWANHVVVASGVWSGMFFKQLGLNNAFLPVKGECLSV-----WNDDIPLTKTLYHDHCYIVPRKSGRLVVGATMKPGD-WSETPDLGGLESVMKKAKTMLPAIQNMK--VDRFWAGLRPGTKDGKPYIGRHPEDSRILFAAGHFRNGILLAPATGALISDLI-----------------------

General information: TITO was launched using: RESULT: Template: 1RYI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1561 47589 30.49 153.02 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 30.49 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_1RYI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RYI-query.scw PDB file : Tito_Scwrl_1RYI.pdb: