Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF 4UW4 Chain:A ((4-136)) ---VPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF

General information: TITO was launched using: RESULT: Template: 4UW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 670 -73638 -109.91 -553.67 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -109.91 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.754

(partial model without unconserved sides chains): PDB file : Tito_4UW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UW4-query.scw PDB file : Tito_Scwrl_4UW4.pdb: