Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPG-ETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKR--KGHILELLTEVTRRMA----EAELVQEGKARKTNPEIQSTLRKRLYLQ 4QUD Chain:A ((63-275)) ---------------------------SRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVC---VLLSHGEEGIIFGTNGP-VDLKKIFNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIET--------------HKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYF-

General information: TITO was launched using: RESULT: Template: 4QUD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 884 -56070 -63.43 -286.07 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -63.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_4QUD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QUD-query.scw PDB file : Tito_Scwrl_4QUD.pdb: