Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHY 1BLK Chain:A ((14-95)) WFFRTISRKDAERQLLAPMNKAGSFLIRESESNKGAFSLSVKDITTQGEVVKHYKIRSLDNGGYYISPRITFPTLQALVQHY

General information: TITO was launched using: RESULT: Template: 1BLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 311 -39975 -128.54 -487.50 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -128.54 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_1BLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BLK-query.scw PDB file : Tito_Scwrl_1BLK.pdb: