Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFPSEQEQIEKFEKDHVAQHYFEVLRTLI---SKKSVFAQQVGLKEVANYLGE-IFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGL-ELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTVFDALEV--PMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE 4MMO Chain:A ((3-434)) -----------------------EELYTLIEFLKKPSISATGEGIDETANYLKETVEKLLGVKANLEKTKGHPVVYAEINVNA--KKTLLIYNHYDVQPVDPISEWKRAPFSATIENDRIYARGASDNKGTLMARLFAI-KHLLDKNELNVNVKLLYEGEEEIGSVNLEDYIEKNTNKLK-ADSVIMEGAGLDPKGRPQIVLGVKGLLYVELVLDYGTKDLHSSNAPLVRNPCIDLAKIISTLVDMGGRVLIEGFYDDVRELTEEERELIKKY-DIDVEELKKALGFKELKYNEKEKIA--EALLTYPTCNVDGFECGYTGKGSKTIVPHRAFAKLDFRLVPNQDPYKVFELLKKHLQKAGFNGEILAHGF-EYPVRTSVNSTVVKAMIESAKKVYGTEPQVIPNSAGTQPMGLFVYKLGIRDAVSAIGAGGYYSNAHAPNENIKIDDYYKAIKHTEEFLKLY-

General information: TITO was launched using: RESULT: Template: 4MMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2291 78311 34.18 184.26 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 34.18 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_4MMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MMO-query.scw PDB file : Tito_Scwrl_4MMO.pdb: