Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKSMVAREAKRQKIVDRYAEKRAALKA------AGDYE------GLSKLPRNASPTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW 5ME1 Chain:N ((2-101)) -AKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW

General information: TITO was launched using: RESULT: Template: 5ME1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 237 25987 109.65 295.31 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain N : 0.80 3D Compatibility (PKB) : 109.65 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_5ME1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ME1-query.scw PDB file : Tito_Scwrl_5ME1.pdb: