Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINSYYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVARFAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYL-KDGALDMEGLRAKLTDKVKFVSLAHASNVLGVVNPIKEIT-QLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFAFSAHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPNMAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVIAFNLGDLH-PHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDKLVDALQKTKEFFNGTF 5VPR Chain:A ((9-413)) MFDIQEIRSQFPILQEKVNGKDLVYLDNAATSQKPKMVLDAINNYYEHYNANVHRGIHTLSQVATEMMEDARKKVQRFINAKHDYEVLFTKGTTEGINLVAYAMTDLIKKDDEIIISY----SNIVPWQMLCQRTGAKLRVIPMNEDGTLQIDVLDEWLSEKTKLVSVNQVSNALGIVNPIDEIIRKVRAKSNAFIFIDGAQAAPHFEIDVQTMDCDFFAFSGHKMYGPTGTGILYGKASVLEQLN---------------------LPFRFEAGTPNIAGNIAIGTAVDFMEKVGRSNIAAHEHALLEYAQRKLLEIEGLKVYGEK--ANRAGVVSFNLSGIGIASDVGMILDKLGIAVRT---------------GTVRASFAVYNTFEEIDILTEGVKKAQKML----

General information: TITO was launched using: RESULT: Template: 5VPR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2032 -87343 -42.98 -241.28 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -42.98 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_5VPR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VPR-query.scw PDB file : Tito_Scwrl_5VPR.pdb: