Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGGRLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQL-AQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAAQWPLEKKKDLASQVLDNNGNQNQL---LNQVHILLEGGRQDDRD 4TTQ Chain:A ((19-204)) ----VGLTGNIASGKSTVAEFFSELGINVIYADKIAKELTSKNTPCYQDIISHFGSSVVLNNGELDRKRIRDIIFSNSNERLWLESLLHPVIRKK---IEEQLIVCTSPYCLIEIPLLFNKHHYPYLQKVLLVIAPLESQLDRIVKRDHCTKKQALAILATQPNLEQRLEAADDVLINESGLSELKAKVNKLH------------

General information: TITO was launched using: RESULT: Template: 4TTQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 729 -58388 -80.09 -320.81 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -80.09 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_4TTQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TTQ-query.scw PDB file : Tito_Scwrl_4TTQ.pdb: