Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFNHVYFGYDENR-PVLKDITCSIFKGQKIAFVGPSGSGKSTIVRLLEQFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGAKPKTSNS 2HYD Chain:A ((340-487)) IDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYDVTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLG-RPTATDEEVVEAAKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLS--

General information: TITO was launched using: RESULT: Template: 2HYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 510 9292 18.22 63.21 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 18.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_2HYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HYD-query.scw PDB file : Tito_Scwrl_2HYD.pdb: