Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFATSQVNPLTNSSVRNLRPKQRRLFMFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITAKLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLNTISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGLNDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL 2ACV Chain:A ((85-157)) ----------------------------------------------------------------------------------------------------------------------------------------------------------LKSPEF----YILTFLESLIPHVKATIKTILSNKVVGLVLDFFCVSMIDVGNEF----GIPSYLFLTSNVGFLSLMLSLKN-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ACV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 156 -52499 -336.53 -719.16 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.48 3D Compatibility (PKB) : -336.53 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_2ACV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ACV-query.scw PDB file : Tito_Scwrl_2ACV.pdb: